Bamboo NiR gene is associated with regeneration capacity

It was reported that the activity of NiR (nitrite reductase) enzyme encoded by the NiR gene was correlated with the regeneration ability in rice. To testify the function of NiR gene in bamboo, seven bamboo species whose calli had different differentiation rate were chosen to analyse their NiR enzyme activity. The results showed that bamboo NiR enzymatic activity had a certain correlation with the regeneration capacity. A NiR gene named DhNiR from Dendrocalamus hamiltonii with high regeneration capacity was cloned. Sequence analysis revealed that the ORF of DhNiR was 1779bp encoding 592 amino acids. Overexpression of DhNiR in rice reduced the time of shoot differentiation and increased the transformation rate, suggesting that DhNiR might play an important role in the regeneration ability of bamboo, and can be applied in regeneration and gene transformation of bamboo and other plant species.     

松南结缕草成熟胚愈伤组织的诱导和再生

松南结缕草成熟种子经灭菌和打破休眠后,接种于含有不同浓度2,4-D的MS和N6培养基上进行愈伤组织诱导。结果表明,结缕草种子的休眠主要在于破除种壳。2,4-D浓度在2~5 mg/L对愈伤组织诱导率无明显影响。基本培养基MS和N6诱导效果相近,初生愈伤组织为白色、半透明,质地松软、水浸状。继代培养基中添加一定浓度的甘露醇、麦芽糖和ABA以及延长继代时间有助于愈伤组织的胚性化。基本培养基MS更适于结缕草初生愈伤组织致密胚性化。胚性愈伤组织在MS添加0.1 mg/L 2,4-D培养基上即可分化出大量具有根和茎叶的再生植株。     

产苦马豆素疯草内生真菌Undifilum oxytropis原生质体的制备和再生

以产苦马豆素内生真菌Undifilum oxytropis NX-FEL001为供试菌株,研究了培养方式、菌龄、酶解时间和酶解温度等,对U.oxytropis原生质体制备和再生的影响。结果表明,U.oxytropis菌丝在质量分数为10g/L纤维素酶+10g/L蜗牛酶+1g/L溶壁酶组成的复合酶溶液,35℃恒温,80r/min振荡孵育3h左右,原生质体释放量最高;原生质体在YCM培养基上,22℃培养9d可以再生形成菌落,培养15d其再生率可达8.5%;原生质体在YCM培养基上再生后其菌落颜色与野生型U.oxytropis不一致,呈现白色;菌丝形态与野生型U.oxytropis相一致,经检测再生后菌丝仍然能够产生苦马豆素。U.oxytropis原生质体的制备,将为进行疯草内生真菌的遗传转化和基因操作研究提供重要工具,为进一步开展疯草内生真菌合成苦马豆素的分子调控机制奠定了基础。     

The Expression of Long Noncoding RNA (H19) in Mouse Skeletal Muscle Development

This experiment was intend to study the changes of long non-coding RNA (H19) expression levels in skeletal muscle development and regeneration,and lay the foundation of its mechanism reach in skeletal muscle development.C2C12 cell line and ICR mice were used as experimental material,bioinformatics assay was used to exploit its non-coding character and low conservatism in different species,and the expressions of H19 in C2C12 cell differentiation,skeletal muscle development and the phase of muscle regeneration were detected by quantitative Real-time PCR (qRT-PCR).The results showed that,H19 expression levels in postnatal mouse skeletal muscle decreased with increasing age;during C2C12 cell differentiation,H19 mRNA increased gradually,and then maintained a high level;The expression of H19 was maintained at a high level through days 4 to 6 after injury.In consideration of its express character in C2C12 cell differentiation and skeletal muscle damage repair model,H19 may play an important role in promoting myogenesis and skeletal muscle regeneration.     

Wnt/β-catenin信号通路上游基因在山羊绒周期性再生及着色过程中的表达分析

为初步探究Wnt/β-catenin信号通路是否参与山羊绒周期性再生及着色过程,本试验采用实时荧光定量技术对Wnt/β-catenin信号通路上游基因β-catenin、Lef1/Tcf3及该通路拮抗基因Dkk1mRNA在白色绒山羊绒毛生长不同时期及黑、白色绒山羊绒毛生长旺盛期时体侧部皮肤组织中的相对表达量进行研究。结果显示,上述通路中β-catenin、Lef1/Tcf3基因mRNA在白色绒山羊不同时期体侧部皮肤组织中的相对表达量具有明显变化规律即生长前期缓慢上调,旺盛期时表达量最高,生长后期及退行期逐渐下调,休止期表达量最低;Dkk1基因mRNA在白色绒山羊不同时期体侧部皮肤组织中的相对表达量则无明显规律性。上述各基因mRNA在黑、白色绒山羊生长旺盛期时体侧部皮肤组织中的相对表达量并无显著差异(P0.05)。结果提示,Wnt/β-catenin信号通路参与山羊绒周期性再生过程,但该通路并不涉及生长旺盛期时绒毛的着色过程。     

Brazilian savanna re-establishment in a monoculture forest:diversity and environmental relations of native regenerating understory in Pinus caribaea Morelet. stands

In this paper we analyze and compare natural regeneration in shrub-tree community in three areas below Pinus caribaea stands and a natural area (cerrado sensu stricto). We also analyze the influence of...     

沙地樟子松人工林冠下人工更新研究

在渐伐后郁闭度不足0.4的沙地樟子松人工林冠下进行人工更新试验,10年后观测各树种的生长状况,并对林下的土壤进行调查.结果表明:利用这种冠下人工更新方式使樟子松纯林转变为多树种混交的复层林;色木槭等4个树种可以作为冠下更新的主培树种;冠下人工更新可以逐渐改良土壤,延缓樟子松人工林林地土壤的衰退.     

DPF喷油助燃装置工作参数对再生过程的影响

以喷油助燃再生方式为例，基于柴油机微粒捕集器（DPF）过滤体孔道热再生模型，对喷油助燃再生装置在不同工作参数下即喷油压力、喷油率、补气量等单因素变化时对再生过程过滤体排气背压、孔道内微粒层厚度及壁面峰值温度等变化规律进行了研究与分析.结果表明：燃烧器的工作参数喷油压力、喷油率、补气量等对再生过程有显著影响.随着喷油压力的提高，过滤体孔道再生壁面峰值温度升高，加快再生速率提高喷油率可加大燃烧器的功率，再生过程壁面峰值温度增幅较大，从过滤体的安全耐热温度来看应加以控制，但喷油率提高到一定程度后，使排气中氧含量不足，燃烧器燃烧效率下降，且影响微粒的氧化速度，导致再生速率随之下降.适当补气可增加排气中氧含量，可加快再生过程，但进一步加大补气量后由于对流传热散失作用加强将减缓再生过程.     

大花萱草新品种“御衣黄”组织培养及植株再生

以大花萱草“御衣黄”花蕾为外植体建立其组织培养再生体系.研究结果表明,大花萱草“御衣黄”花蕾经75％乙醇30 s +0.1％升汞10 min处理后可获得较为理想的灭菌效果；诱导培养基MS+ 6-BA 5 mg/L+ NAA 5mg/L是花蕾理想的脱分化培养基,愈伤组织诱导率达到75％；在降低了激素质量浓度的培养基MS+...     

Analysis of micro-climate on the programs of urban infrastructure regeneration in J city,Republic of Korea

The purpose of this study is to simulate urban micro-climate change due to the variation of wind flow and temperature assuming situations before and after applying programs using urban infrastructure regeneration technologies (UIRTs), and to then quantitatively estimate effectiveness on the urban regeneration schems. UIRTs focus on improving the recycling efficiencies of reclaimed water and biogas produced and to enforce the linkage among all environmental infrastructures associated with water, waste, and energy in an urban block. To implement the micro-climate analyses, data such as land use, topography, building height and materials, and weather conditions were collected, and the results estimated based on fluid simulations of cold wind and temperature variation pathways. There were three programs used in the urban regeneration project in J city: river restoration, traditional market revitalization, and a primary school eco-school. The height of cold wind from the ground level was significantly elevated, and the maximum wind velocity differential between before and after increased by 0.12 m/s due to the expansion of the waterfront space and grassland through the three programs. Overall, a temperature at the ground level dropped by 1.6 °C. Based on these results, the three programs improved the urban environment at ground level, and might ultimately be capable of diminishing the urban heat island effect and mitigating the energy consumption of urban areas.